Purification of extracellular lipase from Pseudomonas aeruginosa.


Lipase (triacylglycerol acylhydrolase, EC was excreted by Pseudomonas aeruginosa PAC1R during the late logarithmic growth phase. Characterization of cell-free culture supernatants by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed the presence of significant amounts of lipopolysaccharide, part of which seemed to be tightly bound… (More)


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