Purification of embryonic rat motoneurons by panning on a monoclonal antibody to the low-affinity NGF receptor.

@article{Camu1992PurificationOE,
  title={Purification of embryonic rat motoneurons by panning on a monoclonal antibody to the low-affinity NGF receptor.},
  author={William Camu and C. E. Henderson},
  journal={Journal of neuroscience methods},
  year={1992},
  volume={44 1},
  pages={59-70}
}
Available methods for purifying motoneurons to homogeneity from rodent spinal cord involve retrograde labelling and fluorescence-activated cell sorting, making them costly and time consuming. Motoneurons are the only neurons within the 15-day embryonic rat spinal cord to express the p75 low-affinity NGF receptor and we show that monoclonal antibody 192-IgG, which binds to the extracellular domain of p75, selectively labels a sub-population of large multipolar ventral spinal cord neurons in… CONTINUE READING

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