Purification of effector-target protein complexes via transient expression in Nicotiana benthamiana.

@article{Win2011PurificationOE,
  title={Purification of effector-target protein complexes via transient expression in Nicotiana benthamiana.},
  author={Joe Win and Sophien Kamoun and Alexandra M. E. Jones},
  journal={Methods in molecular biology},
  year={2011},
  volume={712},
  pages={
          181-94
        }
}
Effectors of plant pathogens play important roles in not only pathogenesis but also plant immunity. Plant pathogens use these effectors to manipulate host cells for colonization, and their activities likely influence the evolution of plant immune responses. Analyses of genome sequences revealed that oomycete pathogens, such as Phytophthora spp., possess hundreds of RXLR effectors that are thought to be delivered into the host cells and hence function inside the cells by interacting with the… 
Protein-Protein Interaction Assays with Effector-GFP Fusions in Nicotiana benthamiana.
TLDR
A five-step pipeline that is suitable for rapid, cost-effective, and medium-throughput screening of pathogen effectors in planta is described.
Candidate Effector Proteins of the Rust Pathogen Melampsora larici-populina Target Diverse Plant Cell Compartments.
TLDR
Data enable us to validate effector proteins from M. larici-populina and reveal that these proteins may target multiple compartments and processes in plant cells, and shows that N. benthamiana can be a powerful heterologous system to study effectors of obligate biotrophic pathogens.
Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
TLDR
The PexRD12/31 family of RXLR-WY effectors, which associate and co-localize with components of the vesicle trafficking machinery, are characterized, indicating that the pathogen may enhance endosomal trafficking during infection.
Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process
TLDR
This study characterized the PexRD12/31 family of RXLR-WY effectors, which associate and colocalize with components of the vesicle trafficking machinery, and identified the host processes targeted by the RXLR class of host-translated effectors of the potato blight pathogen Phytophthora infestans.
Cell re-entry assays do not support models of pathogen-independent translocation of AvrM and AVR3a effectors into plant cells
TLDR
It is found that the secreted fusion proteins do not re-enter plant cells from the apoplast and that the assay is prone to false-positives, so a cautionary note is emitted on the use of the cell re-entry assay for protein trafficking studies.
Rust fungal effectors mimic host transit peptides to translocate into chloroplasts
TLDR
It is shown that the CTP1 transit peptide is necessary and sufficient for accumulation in the stroma of chloroplasts, and is cleaved after translocation.
Oomycetes, effectors, and all that jazz.
Deployment of the Burkholderia glumae type III secretion system as an efficient tool for translocating pathogen effectors to monocot cells.
TLDR
Bacterial T3SS-enabled eukaryotic effector delivery and subcellular localization assays provide a useful method for identifying and studying effector functions in monocot plants.
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