Purification of diphtheria toxin receptor from Vero cells.

@article{Mekada1991PurificationOD,
  title={Purification of diphtheria toxin receptor from Vero cells.},
  author={E Mekada and Hachiro Senoh and Ryo Iwamoto and Yukihiko Okada and Takafumi Uchida},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 30},
  pages={20457-62}
}
Diphtheria toxin receptor has been solubilized from Vero cell membranes with octyl beta-D-glucoside. CRM197, the product of a mutated diphtheria toxin gene, was used for the identification of the receptor. The binding activity of the solubilized receptor was assayed by precipitating the receptor with acetone in the presence of phospholipids and carrier… CONTINUE READING