Purification of cytoplasmic aldehyde dehydrogenase by covalent chromatography on reduced thiopropyl-Sepharose 6B.

Abstract

Disulfiram-modified cytoplasmic aldehyde dehydrogenase binds covalently to the thiol groups of reduced thiopropyl-Sepharose 6B under conditions in which mitochondrial aldehyde dehydrogenase does not bind. After washing the resin, the uncontaminated form of the enzyme is eluted by dithiothreitol solution.

Cite this paper

@article{Kitson1982PurificationOC, title={Purification of cytoplasmic aldehyde dehydrogenase by covalent chromatography on reduced thiopropyl-Sepharose 6B.}, author={Trevor M Kitson}, journal={Journal of chromatography}, year={1982}, volume={234 1}, pages={181-6} }