Purification of cytochrome b558 from bovine polymorphonuclear neutrophils.

  title={Purification of cytochrome b558 from bovine polymorphonuclear neutrophils.},
  author={F. Morel and P. Vignais},
  journal={Biochemical and biophysical research communications},
  volume={149 1},
  • F. Morel, P. Vignais
  • Published 1987
  • Chemistry, Medicine
  • Biochemical and biophysical research communications
A 110 fold purification of cytochrome b558 from resting bovine neutrophils has been achieved. The plasma membrane bound cytochrome b was extracted with aminoxide WS35, a non ionic detergent. The purification procedure included liquid column chromatography on CM-C50 Sephadex, chromatofocusing on the anion exchanger PBE94, and gel filtration on P30 Biogel. The purified preparation was characterized by an heme to protein (nmol/mg) ratio of 7.7. The isoelectric point of cytochrome b was at pH 6.5… Expand
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