Purification of cultured primary rat hepatocytes using selection with ricin A subunit.

@article{Johnston1994PurificationOC,
  title={Purification of cultured primary rat hepatocytes using selection with ricin A subunit.},
  author={David E. Johnston and R R Jasuja},
  journal={Hepatology},
  year={1994},
  volume={20 2},
  pages={
          436-44
        }
}
We had found several methods of hepatocyte isolation to be inadequate for removing Kupffer and endothelial cells and so developed a method of selectively killing these nonparenchymal cells in hepatocyte cultures using the known selective uptake and toxicity of the protein synthesis inhibitor ricin and its A chain by nonparenchymal cells. Kupffer cells were quantitated with the Ku-1 monoclonal antibody. Endothelial and Kupffer cells were identified by means of fluorescence microscopy after… CONTINUE READING

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