Purification of class I medullipins from the venous effluent of isolated normal kidneys perfused under high pressure with saline.

@article{Brooks1994PurificationOC,
  title={Purification of class I medullipins from the venous effluent of isolated normal kidneys perfused under high pressure with saline.},
  author={B. Brooks and L. W. Byers and E. Muirhead and M. Muirhead and J. Pitcock and K. Maddipati and K. Maxey},
  journal={Blood pressure},
  year={1994},
  volume={3 6},
  pages={
          407-17
        }
}
Medullipin I (Med I) is a vasodepressor prohormone which is continuously elaborated into the renal venous effluent (RVE) of isolated rat kidneys perfused under high pressure. We have improved the yield of Med I by substituting saline for the albumin perfusate previously reported; and considerably improved refinement by directly fractionating the crude lipid extract of the RVE with high pressure liquid chromatography. The results show that Med I, as defined by previous physiologic and… Expand
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References

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TLDR
The cytochrome P-450 enzyme system is involved in two major metabolic steps of the medullipin system: synthesis of medullIPin I by the kidney and conversion of medULLipin I to medullips II by the liver as shown previously. Expand
Derivation of antihypertensive neutral renomedullary lipid from renal venous effluent.
TLDR
The ANRL was derived from the renal venous effluent as the kidney exerted its nonexcretory antihypertensive function by virtue of improvement in the extraction of ANRL from fresh renal medulla and the fact that purified ANRL caused an acute vasodepressor effect (acted as a vasodilator). Expand
Renal and circulatory effects of medullipin I, as studied in the in-vivo cross-circulated isolated kidney and intact Wistar-Kyoto (WKY) rat.
TLDR
The view that Med I not only has important and long-lasting depressor effects but also affects renal function in important ways, inducing vasodilatation and increasing GFR, RPF, diuresis and sodium-osmolar excretion is supported. Expand
SECRETION OF MEDULLIPIN I BY ISOLATED KIDNEYS PERFUSED UNDER ELEVATED PRESSURE
1. Medullipin I (Med I) is a hormone extracted from renal papillae and its renomedullary interstitial cells (RIC). Med I is stimulated by elevation of the renal artery perfusion pressure.
Reversal of hypertension by transplants and lipid extracts of cultured renomedullary interstitial cells.
TLDR
The lowering of the hypertensive pressure before there was vascularization of the transplant appears to support the view that the transplanted cells secreted and/ or liberated an antihypertensive substance(s) that seeped out and was absorbed by nearby capillaries and/or lymphatics and circulated and acted in the manner of a hormone. Expand
The renal antihypertensive endocrine function: its relation to cytochrome P-450.
TLDR
It is reasoned that inhibition of the first two pathways of arachidonic acid metabolism potentiates the third pathway, the cytochrome P-450 pathway, which potentifies the antihypertensive function of the kidney. Expand
Biologic contrasts between medullipin I and vasoactive glyceryl compounds.
TLDR
It was concluded that Med I is neither HAG, APRL, SA, nor MO, which causes a delayed onset depressor response when injected intravenously into rats. Expand
Renal vasodepressor mechanisms: the medullipin system
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TLDR
Lowering blood pressure when given by mouth Medullipin I lowers the blood pressure of spontaneously hypertensive rats whengiven by mouth without altering cardiac output or heart rate, supporting its actions as a vasodilator that suppresses sympathetic tone. Expand
Degranulation of Renomedullary Interstitial Cells During Reversal of Hypertension
SUMMARY It was demonstrated earlier that the renal renews effluent of one-kidney, one clip hypertensive rats contained a vasodepressor Upid resembling the antihypertenslve neutral renomedullary llpldExpand
Derivation of neutral antihypertensive lipid from renal venous effluent in rats.
TLDR
A deficiency of the secretion of the antihypertensive hormone may play a role in the pathogenesis of the one-kidney, one-clip hypertensive model. Expand
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