Purification of chicken intestinal receptor for 1,25-dihydroxyvitamin D.

@article{Pike1979PurificationOC,
  title={Purification of chicken intestinal receptor for 1,25-dihydroxyvitamin D.},
  author={John Wesley Pike and Mark R. Haussler},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1979},
  volume={76 11},
  pages={5485-9}
}
The 3.3S chicken intestinal receptor for 1,25-dihydroxyvitamin D [1,25-(OH)2D] has been purified approximately 86,000-fold from the cytosolic fraction. The receptor was selectively precipitated with Polymin P from high-speed supernatants derived from 800 g of intestinal mucosa and then sequentially chromatographed on DNA-cellulose. Sephacryl, blue dextran-Sepharose, DNA-cellulose, and heparin-Sepharose. Polyacrylamide gel electrophoresis of 8-10 microgram of the purified receptor in sodium… CONTINUE READING

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