Purification of cathepsin D from rabbit skeletal muscle and its action towards myofibrils.

@article{Okitani1981PurificationOC,
  title={Purification of cathepsin D from rabbit skeletal muscle and its action towards myofibrils.},
  author={Akihiro Okitani and Takashi Matsumoto and Yukiko Kitamura and Hiroaki Kato},
  journal={Biochimica et biophysica acta},
  year={1981},
  volume={662 2},
  pages={202-9}
}
Cathepsin D (EC 3.4.23.4) was purified from rabbit skeletal muscle using acetone-dried muscle powder as starting material. After the acetone-dried powder was extracted with 0.2 mM ATP, the extract was fractionated with acetone an subjected to DEAE-Sephadex A-50 and Sephadex G-100 column chromatography. Rechromatography on a Sephadex G-100 column resulted in… CONTINUE READING