Purification of carbonic anhydrase isoenzymes by high-performance affinity chromatography and hydrophobic interaction chromatography.

Abstract

Isoenzymes of carbonic anhydrase were purified by a combination of affinity chromatography and hydrophobic interaction chromatography. Immobilization of sulfonamides on an epoxy-activated support provided a stationary phase for affinity chromatography which was stable to hydrolysis by carbonic anhydrase. A first purification step allowed the isolation of… (More)

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@article{AbouRebyeh1991PurificationOC, title={Purification of carbonic anhydrase isoenzymes by high-performance affinity chromatography and hydrophobic interaction chromatography.}, author={Hassan Abou-Rebyeh and Djuro Josi{\'c} and Klaus Gottschall and K Schubert-Rehberg and Florian Koerber}, journal={Journal of chromatography}, year={1991}, volume={566 2}, pages={351-9} }