Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

@article{Aviv1972PurificationOB,
  title={Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.},
  author={H. Aviv and Philip Leder},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1972},
  volume={69 6},
  pages={1408-12}
}
A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologically active rabbit globin mRNA has been purified by this procedure and assayed for its ability to direct the synthesis of rabbit globin in a cell-free extract of ascites tumor. Inasmuch as various… CONTINUE READING
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