Purification of bacteriophage T7 DNA-membrane complex and its application to the in vitro recombination reaction.

@article{Shimizu1985PurificationOB,
  title={Purification of bacteriophage T7 DNA-membrane complex and its application to the in vitro recombination reaction.},
  author={Kuniyoshi Shimizu and Hiromasa Araki and Hideyuki Ogawa},
  journal={Journal of biochemistry},
  year={1985},
  volume={98 6},
  pages={1473-85}
}
In order to construct an in vitro recombination system of T7 DNA, the reaction products of which resemble those in vivo in structure, T7 DNA-membrane complex which is free from concomitant DNase activity was purified from T7 phage-infected cells. T7-infected cells were lysed with T4 lysozyme/Brij58, and T7 DNA-membrane complex was purified through three… CONTINUE READING