Purification of antibodies using affinity chromatography.

  title={Purification of antibodies using affinity chromatography.},
  author={Elaine Darcy and P. L{\'e}onard and Jenny Fitzgerald and Martin Danaher and Richard O'Kennedy},
  journal={Methods in molecular biology},
Affinity chromatography permits the isolation of a target analyte from a complex mixture and can be utilised to purify proteins, carbohydrates, drugs, haptens, or any analyte of interest once an affinity pair is available. It involves the exploitation of specific interactions between a binding affinity pair, such as those between an antibody and its associated antigen, or between any ligand and its associated binding receptor/protein. With the discovery of protein A in 1970, and, subsequently… CONTINUE READING

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