Purification of an oligonucleotide at high column loading by high affinity, low-molecular-mass displacers.

@article{Tugcu2001PurificationOA,
  title={Purification of an oligonucleotide at high column loading by high affinity, low-molecular-mass displacers.},
  author={Nihal Tugcu and Rajendra R Deshmukh and Y S Sanghvic and J A Moored and Steven M. Cramer},
  journal={Journal of chromatography. A},
  year={2001},
  volume={923 1-2},
  pages={65-73}
}
The development of efficient techniques for large-scale oligonucleotide purification is of great interest due to the increased demand for antisense oligonucleotides as therapeutics as well as their use for target validation and gene functionalization. This paper describes the use of anion-exchange displacement chromatography for the purification of 20-mer… CONTINUE READING