Purification of an acidic deoxyribonuclease II from the primate spleen

@article{Pitout1974PurificationOA,
  title={Purification of an acidic deoxyribonuclease II from the primate spleen},
  author={Marthinus Pitout and P. G. Kempff and Johannes C. Schabort},
  journal={International Journal of Biochemistry},
  year={1974},
  volume={5},
  pages={241-246}
}
View via Publisher
6 Citations

6 Citations

Lysosomal acid deoxyribonuclease from vervet monkey livers--I. Purification and physico-chemical characterization.
Mechanism of action of deoxyribonuclease II from human lymphoblasts.
TLDR
Application of DNA sequencing techniques has further revealed a unique resistance of 3' termini to hydrolysis by this enzyme, and the presence of 3'-phosphoryl termini on the DNA cleavage products was confirmed since they failed to function as primers for DNA synthesis catalyzed by Escherichia coli DNA polymerase I.
Deoxyribonuclease II purified from the isolated lysosomes of porcine spleen and from porcine liver homogenates. Comparison with deoxyribonuclease II purified from porcine spleen homogenates.
Mammalian nucleolytic enzymes.
Partial purification of deoxyribonuclease II from human spleen.
DNase II in bull and ram sperm tail and mitochondria.
TLDR
Extracts of bull and ram sperm tails prepared by DTT and CTAB treatment contained deoxyribonuclease II, degrading only native double-stranded DNA at acid pH (3.9--4.5).

References

SHOWING 1-10 OF 21 REFERENCES
Studies on acid hydrolases. IV. Isolation and characterization of spleen exonuclease.
Salmon testes deoxyribonuclease: large-scale purification and properties.
Separation and partial characterization of three acid deoxyribonucleases of the hepatopancreas of Octopus vulgaris.
Purification and some properties of an acid deoxyribonuclease from testes of chinook salmon Oncorhynchus tshawytscha.
11 Spleen Acid Deoxyribonuclease
Bond-cleaving specificity of a deoxyribonuclease II from mouse mammary tumors.
A comparative study of acid deoxyribonucleases extracted from different tissues and species.
TLDR
The results obtained strongly indicate that the enzymatic activity is associated with protein molecules endowed with very similar physical, chemical, and enzymic properties.
Determination of glycogen with the anthrone reagent.
[39] Analytical disc gel electrophoresis
Protein measurement with the Folin phenol reagent.
...
...