Purification of an SOS repressor from Bacillus subtilis.

@article{Lovett1993PurificationOA,
  title={Purification of an SOS repressor from Bacillus subtilis.},
  author={Charles M. Lovett and Kwang J. Cho and Thomas M. O'gara},
  journal={Journal of bacteriology},
  year={1993},
  volume={175 21},
  pages={
          6842-9
        }
}
We have identified in Bacillus subtilis a DNA-binding protein that is functionally analogous to the Escherichia coli LexA protein. We show that the 23-kDa B. subtilis protein binds specifically to the consensus sequence 5'-GAACN4GTTC-3' located within the putative promoter regions of four distinct B. subtilis DNA damage-inducible genes: dinA, dinB, dinC, and recA. In RecA+ strains, the protein's specific DNA binding activity was abolished following treatment with mitomycin C; the decrease in… CONTINUE READING
BETA

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