Purification of an Escherichia coli serogroup O157:H7 verotoxin and its detection in North American hemorrhagic colitis isolates.

Abstract

Verotoxin (VT), which is immunologically unrelated to VT1 (Shiga-like toxin I), was purified from the culture filtrate of Escherichia coli hemorrhagic colitis serogroup O157:H7 strain 3657 by copper ion chelate affinity chromatography followed by anion-exchange chromatography. The isoelectric point by sucrose density gradient isoelectric focusing was 5.0… (More)

Topics

Cite this paper

@article{Dickie1989PurificationOA, title={Purification of an Escherichia coli serogroup O157:H7 verotoxin and its detection in North American hemorrhagic colitis isolates.}, author={N H Dickie and Jamie Speirs and M Akhtar and Wynona M Johnson and Rita Szab{\'o}}, journal={Journal of clinical microbiology}, year={1989}, volume={27 9}, pages={1973-8} }