Purification of adenoviral vectors by combined anion exchange and gel filtration chromatography.

@article{Eglon2009PurificationOA,
  title={Purification of adenoviral vectors by combined anion exchange and gel filtration chromatography.},
  author={Marc N Eglon and Aoife M Duffy and Timothy O'Brien and Padraig M. Strappe},
  journal={The journal of gene medicine},
  year={2009},
  volume={11 11},
  pages={978-89}
}
BACKGROUND Adenoviral vectors are used extensively in human gene therapy trials and in vaccine development. Large-scale GMP production requires a downstream purification process, and liquid chromatography is emerging as the most powerful mode of purification, enabling the production of vectors at a clinically relevant scale and quality. The present study describes the development of a two-step high-performance liquid chromatography (HPLC) process combining anion exchange (AIEX) and gel… CONTINUE READING

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Chromatographic purification of adenoviral vectors on anion-exchange resins.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences • 2015
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