Purification of acute phase anti-hepatitis A virus (HAV) IgM and development of an IgM solid-phase radioimmunoassay for the detection of HAV.

Abstract

A simple two-step procedure for the purification of IgM from acute phase hepatitis A infected chimpanzee serum has been developed. The procedure involves the precipitation of the euglobulin fraction with boric acid and the fractionation of the resuspended material on Sephacryl S-300 (Pharmacia Fine Chemicals, Piscataway, NJ). The purified IgM was used to… (More)

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@article{Fields1982PurificationOA, title={Purification of acute phase anti-hepatitis A virus (HAV) IgM and development of an IgM solid-phase radioimmunoassay for the detection of HAV.}, author={Howard A Fields and Karen A. McCaustland and Daniel W. Bradley and James Maynard}, journal={Journal of immunological methods}, year={1982}, volume={51 2}, pages={149-57} }