Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum.

@article{Matsuyama1992PurificationOA,
  title={Purification of acetyl coenzyme A: deacetylacephalosporin C O-acetyltransferase from Acremonium chrysogenum.},
  author={Kayo Matsuyama and Hanako Matsumoto and Akihiko Matsuda and Hisashi Sugiura and Kazuya Komatsu and Sohsuke Ichikawa},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1992},
  volume={56 9},
  pages={1410-2}
}
Acetyl CoA: deacetylcephalosporin C O-acetyltransferase, which catalyzes the final step of the biosynthetic pathway to cephalosporin C, was stabilized by a buffer solution containing 7-aminocephalosporanic acid and purified over 1300-fold from Acremonium chrysogenum. The purified enzyme has a molecular weight of 55,000 as measured by gel filtration. SDS… CONTINUE READING