Purification of a secreted form of recombinant rabies virus glycoprotein: comparison of two affinity tags.

Abstract

Expression of recombinant eukaryotic proteins in transfected mammalian cell lines has become an important approach for the characterization of the structure and function of these proteins. However, it is often difficult to recover and purify the recombinant proteins. Therefore, the use of fusion proteins incorporating epitope or affinity tags has become… (More)

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