Purification of a regulatory subunit of type II cAMP-dependent protein kinase from Drosophila heads.

@article{Inoue1997PurificationOA,
  title={Purification of a regulatory subunit of type II cAMP-dependent protein kinase from Drosophila heads.},
  author={Hiroko Inoue and Takashi Yoshioka},
  journal={Biochemical and biophysical research communications},
  year={1997},
  volume={235 1},
  pages={223-6}
}
The cytosolic extract from Drosophila heads was separated using anion-exchange column chromatography. Two types of cAMP-dependent protein kinase (PKA), type I and type II, were detected, and type II PKA was found to be a major isozyme. The regulatory subunit of type II PKA (RII) was purified, and only one isoform was observed. The purified protein had an… CONTINUE READING