Purification of a plasma membrane-associated GTPase-activating protein specific for rap1/Krev-1 from HL60 cells.

@article{Polakis1991PurificationOA,
  title={Purification of a plasma membrane-associated GTPase-activating protein specific for rap1/Krev-1 from HL60 cells.},
  author={Paul Polakis and Bonnee Rubinfeld and Tara Evans and Frank Mccormick},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 1},
  pages={239-43}
}
rap1/Krev-1 is a p21ras-related GTP-binding protein that has been implicated in the reversion of the ras-transformed cell phenotype. We have identified a GTPase-activating protein (GAP) specific for rap in plasma membranes isolated from differentiated HL60 cells. The rap GAP activity remained quantitatively associated with the membrane following washes with buffered 1 M LiCl containing 20 mM EDTA but was solubilized with the detergents Nonidet P-40 and deoxycholate. On the basis of size… CONTINUE READING

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