Purification of a phosphate carrier in pig heart mitochondria by affinity chromatography on mersalyl-ultrogel.


Mersalyl is a powerful inhibitor of phosphate transport in mitochondria. In addition, by its reversible reaction with thiol groups, it can protect the latter from irreversible reaction with N-ethylmaleimide [ 1,2]. Use of this property enabled a group of proteins of M, 3.0-3.2 X lo4 to be identified in the internal mitochondrial membrane [3-g]. This group… (More)


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