Purification of a novel phospholipase C isozyme from bovine cerebellum.

  title={Purification of a novel phospholipase C isozyme from bovine cerebellum.},
  author={Do Sik Min and Dong-Myung Kim and Young Han Lee and Jeong Kon Seo and Pann Ghill Suh and Sung Ho Ryu},
  journal={The Journal of biological chemistry},
  volume={268 16},
An isozyme of phosphoinositide-specific phospholipase C (PLC) was purified to near homogeneity from bovine cerebellum by a combination of several column chromatography procedures. Approximately 80 micrograms of pure enzyme were obtained from 4 kg of bovine cerebellum, with a final specific activity of 7.5 mumol/min/mg protein in the presence of 0.1% deoxycholate. The enzyme is specific for phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate but does not hydrolyze phosphatidylcholine… CONTINUE READING