Purification of a novel phospholipase A2 from bovine seminal plasma.

  title={Purification of a novel phospholipase A2 from bovine seminal plasma.},
  author={S Soubeyrand and Abdelkrim Khadir and Yves Brindle and P. V. Manjunath},
  journal={The Journal of biological chemistry},
  volume={272 1},
Phospholipases A2 are enzymes believed to play important roles in numerous physiological systems including sperm cell maturation. Relatively little work has, however, been devoted to study these enzymes in seminal plasma. We therefore undertook the purification and characterization of this enzyme from bovine seminal plasma. After a 330-fold purification, an activity corresponding to a protein of 100 kDa was identified by gel filtration. SDS-polyacrylamide gel electrophoresis analysis of the… CONTINUE READING