Purification of a factor which provides a costimulatory signal for gamma interferon production.

@article{Nakamura1993PurificationOA,
  title={Purification of a factor which provides a costimulatory signal for gamma interferon production.},
  author={Kayoko Nakamura and Hitoshi Okamura and Kumiko Nagata and Toshinori Komatsu and Teruko Tamura},
  journal={Infection and immunity},
  year={1993},
  volume={61 1},
  pages={64-70}
}
A protein factor which induces high levels of gamma interferon (IFN-gamma) in resting splenic nonadherent cells was isolated from the sera of mice with generalized inflammation caused by endotoxic shock. The factor was highly purified by ammonium sulfate precipitation followed by ion-exchange column chromatography on DEAE-Sepharose, molecular sieving on Ultrogel AcA 44, and hydrophobic column chromatography with phenyl-Sepharose. It was further purified to apparent homogeneity by polyacrylamide… CONTINUE READING

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