Purification of a factor inducing differentiation in murine myelomonocytic leukemia cells. Identification as granulocyte colony-stimulating factor.

@article{Nicola1983PurificationOA,
  title={Purification of a factor inducing differentiation in murine myelomonocytic leukemia cells. Identification as granulocyte colony-stimulating factor.},
  author={Nicos A. Nicola and Donald Metcalf and Misako Matsumoto and Gibbes R. Johnson},
  journal={The Journal of biological chemistry},
  year={1983},
  volume={258 14},
  pages={9017-23}
}
A naturally occurring inducer of terminal differentiation in a murine myelomonocytic leukemia cell line (WEHI-3B) was purified to apparent homogeneity from medium conditioned by lungs from mice injected with bacterial endotoxin. The factor was purified over 400,000-fold by sequential fractionation using salting out chromatography, chromatography on phenyl-Sepharose, gel filtration on Bio-Gel P-60 in 1 M acetic acid, reverse-phase high performance liquid chromatography on a phenyl-silica column… CONTINUE READING

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