Purification of a Membrane-Bound UDP-Glucose:Sterol [beta]-D-Glucosyltransferase Based on Its Solubility in Diethyl Ether.

@article{Warnecke1994PurificationOA,
  title={Purification of a Membrane-Bound UDP-Glucose:Sterol [beta]-D-Glucosyltransferase Based on Its Solubility in Diethyl Ether.},
  author={Dirk C. Warnecke and Ernst Heinz},
  journal={Plant physiology},
  year={1994},
  volume={105 4},
  pages={
          1067-1073
        }
}
Membrane-bound UDP-glucose:sterol [beta]-D-glucosyltransferase (UDPG-SGTase) catalyzes the formation of steryl glucosides from UDP-glucose and free sterols. This enzyme was purified from etiolated oat shoots (Avena sativa L. cv Alfred) in five steps. UDPG-SGTase was solubilized from a microsomal fraction with the detergent n-octyl-[beta]-D-thioglucopyranoside and then extracted into diethyl ether. Subsequent removal of the organic solvent, resolubilization with an aqueous buffer, and two column… CONTINUE READING
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