Purification of a 28 kD non-aggregating tryptic peptide of the Duffy blood group protein.

@article{Waniowska1993PurificationOA,
  title={Purification of a 28 kD non-aggregating tryptic peptide of the Duffy blood group protein.},
  author={K Waśniowska and Patrick Eichenberger and F Kugele and Tricia J. Hadley},
  journal={Biochemical and biophysical research communications},
  year={1993},
  volume={192 2},
  pages={366-72}
}
Duffy blood group antigenic epitopes are located on a 35-43 kD integral membrane protein of the erythrocyte membrane. This protein functions as a receptor for the human malaria parasite, Plasmodium vivax. The Duffy protein has been difficult to purify because of its tendency to form aggregates. Here we describe purification of a 28 kD tryptic fragment of the Duffy protein and purification of an 18 kD de-glycosylated form of the Duffy tryptic peptide using Thiopropyl Sepharose 6B chromatography… CONTINUE READING