Purification of a 190 kDa protein from smooth muscle: relationship to talin.

@article{OHalloran1986PurificationOA,
  title={Purification of a 190 kDa protein from smooth muscle: relationship to talin.},
  author={Theresa J. O'Halloran and Keith Burridge},
  journal={Biochimica et biophysica acta},
  year={1986},
  volume={869 3},
  pages={337-49}
}
Several studies of vinculin-binding proteins have described a 190 kDa protein in chicken gizzard smooth muscle which binds radioiodinated vinculin. We have purified and studied the 190 kDa protein from chicken gizzard smooth muscle. By indirect immunofluorescence, an antiserum raised against the 190 kDa protein stains adhesion plaques (focal contacts), ruffling membranes, and fibrillar streaks on the dorsal and ventral surfaces of fibroblasts. Both the binding to vinculin and the location of… CONTINUE READING