Purification of Total RNA from Human Stool Samples

  title={Purification of Total RNA from Human Stool Samples},
  author={Richard J. Alexander and Robert Franklin Raicht},
  journal={Digestive Diseases and Sciences},
While colonoscopy may detect early-stage colontumors, a less invasive and more costeffective techniquewould be beneficial. Stool, which picks up sloughed-offcolonic epithelial cells, would be ideal for sampling the mucosa; shed tumor cells maydisplay alterations in gene expression observed inintact tumors. It is first necessary, however, to showthat RNA can be isolated from human feces and that this RNA contains human gene transcripts. We havetherefore developed a method for the isolation of… CONTINUE READING


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