Purification of Oligonucleotides Using Denaturing Polyacrylamide Gel Electrophoresis

@article{Ellington2001PurificationOO,
  title={Purification of Oligonucleotides Using Denaturing Polyacrylamide Gel Electrophoresis},
  author={Andrew D. Ellington and Jack D. Pollard},
  journal={Current Protocols in Molecular Biology},
  year={2001},
  volume={42}
}
Cloning vectors derived from filamentous phage are extremely useful because they allow cloned DNA to be isolated as either single‐ or double‐stranded DNA. This unit contains protocols for preparing both forms of DNA and for characterizing inserts in M13‐derived vectors. A protocol is also presented for preparing single‐stranded DNA from plasmids using superinfection with helper phage. This method is advantageous because it allows cloned DNA to be maintained in the form of a plasmid while… 

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We describe the use of polyacrylamide gel electrophoresis to estimate chain lengths of double- and single-stranded DNA molecules in the size range 20-1000 base pairs (or nucleotides). Double-stranded

Identification and suppression of secondary structures formed from deoxy-oligonucleotides during electrophoresis in denaturing polyacrylamide-gels.

Nature and stability of secondary structures formed from DNA-fragments during electrophoresis in urea containing polyacrylamide-gels were investigated. Duplices and especially hairpin loops were

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