Purification of Mycoplasma gallisepticum membrane proteins p52, p67 (pMGA), and p77 by high-performance liquid chromatography.


The plasma membrane of the avian pathogen Mycoplasma gallisepticum contains about 200 polypeptides including the major lipoprotein pMGA. We have developed a simple and efficient procedure for the purification of three membrane proteins of this wall-less bacterium. Proteins were selectively extracted from isolated plasma membranes with the mild zwitterionic… (More)