Purification of Lectin from Micropropagated Roots Derived from Aseptic Seedling of Canavalia ensiformis L.

@article{Amani2011PurificationOL,
  title={Purification of Lectin from Micropropagated Roots Derived from Aseptic Seedling of Canavalia ensiformis L.},
  author={Samreen Amani and Afshin Iram and Anwar Shahzad and K M Neelofar and Aabgeena Naeem},
  journal={International Journal of Peptide Research and Therapeutics},
  year={2011},
  volume={17},
  pages={317-324}
}
A well-organized protocol has been developed for high frequency root germination from the seed of Canavalia ensiformis on Murashige and Skoog (MS) medium. Surprisingly, the seeds that were grown on the MS medium having no growth hormone showed the best response. Roots of 30 days old aseptic seedling were homogenized and a lectin from them was purified on Sephadex G-50 affinity column. The finding that final product is a pure lectin was confirmed by specific hemagglutinating property. The final… CONTINUE READING

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