Purification of HIV-1 virions by subtilisin digestion or CD45 immunoaffinity depletion for biochemical studies.


The presence of cellular proteins outside and inside retroviruses can indicate the roles they play in viral biology. However, experiments examining retroviruses can be complicated by the contamination of even highly purified virion preparations with nonviral particles (either microvesicles or exosomes). Two useful methods have been developed that can remove… (More)
DOI: 10.1007/978-1-59745-170-3_2