Purification of HIV-1 gag virus-like particles and separation of other extracellular particles.


Enveloped virus-like particles (VLPs) are increasingly used as vaccines and immunotherapeutics. Frequently, very time consuming density gradient centrifugation techniques are used for purification of VLPs. However, the progress towards optimized large-scale VLP production increased the demand for fast, cost efficient and scale able purification processes… (More)
DOI: 10.1016/j.chroma.2016.05.053