Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis.

@article{Nopparatana1991PurificationOG,
  title={Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis.},
  author={C Nopparatana and P Setasuban and Wanpen Chaicumpa and Pramuan Tapchaisri},
  journal={International journal for parasitology},
  year={1991},
  volume={21 6},
  pages={677-87}
}
Specific antigen of G. spinigerum which has been shown to be a protein with a relative mol. wt of 24,000 (24K) was prepared from the advanced third-stage larvae (L3) obtained from the livers of naturally infected eels. The L3 were ground and extracted with water. Purification procedures involved gel filtration, chromatofocussing and anion exchange column chromatographies, while characterization of the specific antigen was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis… CONTINUE READING
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