Purification of GST-Tagged Proteins.

  title={Purification of GST-Tagged Proteins.},
  author={Frank Schaefer and Nicole Seip and Barbara Maertens and Helena Block and Jan Kubicek},
  journal={Methods in enzymology},
This protocol describes the purification of recombinant proteins fused to glutathione S-transferase (GST, GST-tagged proteins) by Glutathione Affinity purification. The GST tag frequently increases the solubility of the fused protein of interest and thus enables its purification and subsequent functional characterization. The GST-tagged protein specifically binds to glutathione immobilized to a matrix (e.g., agarose) and can be easily separated from a cell lysate by a bind-wash-elute procedure… CONTINUE READING

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