Purification of GFP fusion proteins with high purity and yield by monoclonal antibody-coupled affinity column chromatography.

@article{Zhuang2008PurificationOG,
  title={Purification of GFP fusion proteins with high purity and yield by monoclonal antibody-coupled affinity column chromatography.},
  author={Ran Zhuang and Yuan Zhang and Rui Zhang and Chaojun Song and Kun Yang and An-gang Yang and Boquan Jin},
  journal={Protein expression and purification},
  year={2008},
  volume={59 1},
  pages={138-43}
}
GFP has often been used as a marker of gene expression, protein localization in living and fixed tissues as well as for protein targeting in intact cells and organisms. Monitoring foreign protein expression via GFP fusion is also very appealing for bioprocess applications. Many cells, including bacterial, fungal, plant, insect and mammalian cells, can express recombinant GFP (rGFP) efficiently. Several methods and procedures have been developed to purify the rGFP or recombinant proteins fused… CONTINUE READING
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