Purification of Extracellular Cholesterol Oxidase with High Activity in the Presence of Organic Solvents from Pseudomonas sp. Strain ST-200

@article{Doukyu1998PurificationOE,
  title={Purification of Extracellular Cholesterol Oxidase with High Activity in the Presence of Organic Solvents from Pseudomonas sp. Strain ST-200},
  author={Doukyu and Aono},
  journal={Applied and environmental microbiology},
  year={1998},
  volume={64 5},
  pages={1929-32}
}
  • Doukyu, Aono
  • Published 1998 in Applied and environmental microbiology
Extracellular cholesterol oxidase of Pseudomonas sp. strain ST-200 was purified from the culture supernatant. This oxidase contained bound flavin and was categorized as a 3beta-hydroxysteroid oxidase, converting 3beta-hydroxyl groups to keto groups. The molecular mass was 60 kDa. The enzyme was stable at pH 4 to 11 and active at pH 5.0 to 8.5, showing optimal activity at pH 7 at 60 degreesC. The Michaelis constant of the ST-200 cholesterol oxidase was lower than those of commercially available… CONTINUE READING