Purification of Candida guilliermondii and Pichia ohmeri killer toxin as an active agent against Penicillium expansum.

Abstract

An antifungal assay with cell-free culture supernatant of Pichia ohmeri 158 and Candida guilliermondii P3 was tested against Penicillium expansum strain #2 at 25 degrees C by measuring hyphal length and percentage conidia germination. C. guilliermondii was more effective against P. expansum conidia germination (58.15% inhibition), while P. ohmeri showed higher inhibition of mycelial growth (66.17%), indicating a probable mechanism associated with killer activity. This killer toxin (molecular mass <3 kDa) was partially purified by normal phase HPLC, using TSKgel Amide-80 analytical and preparative columns. Compared with crude extract, the killer toxin eluted from the post analytical column significantly inhibited P. expansum:% inhibition rose from 42.16 to 90.93% (C. guilliermondii) and 39.32 to 91.12% (P. ohmeri) (p < 0.05). The one-step purification process was adequate in isolating killer toxin from culture supernatant and also increased anti-Penicillium activity.

DOI: 10.1080/02652030802227227

Cite this paper

@article{Coelho2009PurificationOC, title={Purification of Candida guilliermondii and Pichia ohmeri killer toxin as an active agent against Penicillium expansum.}, author={Alexandre Rodrigo Coelho and Masahico Tachi and Fernando Carlos Pagnocca and Gisele Maria Andrade Nobrega and Fernando Leite Hoffmann and Ken-ichi Harada and Elisa Yoko Hirooka}, journal={Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment}, year={2009}, volume={26 1}, pages={73-81} }