Purification of 5'-nucleotidase from human seminal plasma.

  title={Purification of 5'-nucleotidase from human seminal plasma.},
  author={Carlo Fini and Marcello Coli and Aredsio Floridi},
  journal={Biochimica et biophysica acta},
  volume={1075 1},
5'-Nucleotidase from human seminal plasma was purified to electrophoretic homogeneity and some of its kinetic and molecular properties compared with those of 5'-nucleotidase from bull seminal plasma. The purification of the enzyme was achieved by using the same affinity chromatography media (Con A-Sepharose and AMP-Agarose or ADP-Agarose) previously used… CONTINUE READING