Purification of 2-oxo acid dehydrogenase multienzyme complexes from ox heart by a new method.

@article{Stanley1980PurificationO2,
  title={Purification of 2-oxo acid dehydrogenase multienzyme complexes from ox heart by a new method.},
  author={Carolyn Stanley and Richard N. Perham},
  journal={The Biochemical journal},
  year={1980},
  volume={191 1},
  pages={147-54}
}
A new method is described that allows the parallel purification of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multienzyme complexes from ox heart without the need for prior isolation of mitochondria. All the assayable activity of the 2-oxo acid dehydrogenase complexes in the disrupted tissue is made soluble by the inclusion of non-ionic detergents such as Triton X-100 or Tween-80 in the buffer used for the initial extraction of the enzyme complexes. The yields of the pyruvate… CONTINUE READING

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