Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

Abstract

Sar2676, a pantothenate synthetase with a molecular weight of 31 419 Da from methicillin-resistant Staphylococcus aureus, has been expressed, purified and crystallized at 293 K. The protein crystallizes in a primitive triclinic lattice, with unit-cell parameters a = 45.3, b = 60.5, c = 117.6 A, alpha = 87.2, beta = 81.2, gamma = 68.4 degrees . A complete… (More)

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@article{Seetharamappa2007PurificationCA, title={Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.}, author={Jaldappagari Seetharamappa and Muse Oke and Huanting Liu and Stephen A. McMahon and K A Johnson and Lester G. Carter and Mark Dorward and Michal Zawadzki and Ian M. Overton and C A Johannes van Niekirk and Shirley Graham and Catherine H. Botting and Garry L. Taylor and Malcolm F. White and Geoffrey J. Barton and Peter J. Coote and James H. Naismith}, journal={Acta crystallographica. Section F, Structural biology and crystallization communications}, year={2007}, volume={63 Pt 6}, pages={488-91} }