Purification by DNA affinity chromatography of two polypeptides that contact the NF-AT DNA binding site in the interleukin 2 promoter.

Abstract

NF-AT is a lymphoid-specific transcription factor that is though to be largely responsible for determining the cell type-specific expression of the interleukin 2 (IL2) gene. Using two different purification schemes, we found that the NF-AT binding site present in the IL2 promoter is the target for two distinct polypeptides with a relative molecular weight… (More)

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