Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid.

Abstract

recBC DNase of Escherichia coli has been purified from the transformant, HB101/pFS11-04 (recB+ recC+), by successive ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephadex G-150 gel filtration, hydroxyapatite chromatography, DNA cellulose affinity chromatography, and second DEAE-cellulose chromatography. The purified enzyme was obtained in… (More)

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@article{Umeno1985PurificationAS, title={Purification and subunit structure of recBC DNase from Escherichia coli harboring a recB and recC genes-inserted plasmid.}, author={M. Umeno and Motonobu Anai and M. Sasaki and Y. Takagi}, journal={Journal of biochemistry}, year={1985}, volume={98 3}, pages={681-5} }