Purification and structural stability of white Spanish broom (Cytisus multiflorus) peroxidase.

Abstract

New plant peroxidase has been isolated to homogeneity from the white Spanish broom Cytisus multiflorus. The enzyme purification steps included homogenization, NH(4)SO(4) precipitation, extraction of broom colored compounds and consecutive chromatography on Phenyl-Sepharose, HiTrap™ SP HP and Superdex-75 and 200. The novel peroxidase was characterized as… (More)
DOI: 10.1016/j.ijbiomac.2014.09.015

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@article{Galende2015PurificationAS, title={Purification and structural stability of white Spanish broom (Cytisus multiflorus) peroxidase.}, author={Patricia P{\'e}rez Galende and Nazaret Hidalgo Cuadrado and Juan Arellano and Francisco Gavilanes and Eduard Ya Kostetsky and Galina G. Zhadan and Enrique Villar and Manuel G. Roig and John. F. Kennedy and Valery L. Shnyrov}, journal={International journal of biological macromolecules}, year={2015}, volume={72}, pages={718-23} }