Purification and structural identification of an autoinducer for the luminescence system of Vibrio harveyi.

@article{Cao1989PurificationAS,
  title={Purification and structural identification of an autoinducer for the luminescence system of Vibrio harveyi.},
  author={Junning Cao and Edward A. Meighen},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 36},
  pages={
          21670-6
        }
}
An autoinducer required for the growth-dependent development of luminescence in Vibrio harveyi has been purified, structurally identified, and chemically synthesized. The autoinducer, which is excreted by the cells, was extracted with chloroform from conditioned media in which V. harveyi cells had been grown. The concentrated extract was separated on a silica gel column and the autoinducer activity further purified by thin layer, paper, and high performance liquid chromatography. The structure… CONTINUE READING

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